Date: Thu Mar 29 2007 - 02:31:20 EDT
Hi Sunny, to get the very best signal, go with the PE conjugated secundaries instead of the FITC - regardless of manufacturer. As for supplier: we use a lot of Jackson Immunoresearch secundaries, esp. multi-species (typically bovine, human) crossabsorbed goat anti mouse Fab2's. (http://www.jacksonimmuno.com/) I never did a head-to-head comparison with DAKO products, so this is no endorsement of the former supplier's superiority but rather a suggested alternative, which is what you seem to be looking for. Best of luck, Guy Next generation therapeutic <http://www.ablynx.com/> antibodies Guy Hermans, PhD Senior Scientist Ablynx NV Technologiepark 4 B-9052 Zwijnaarde Belgium guy.hermans@ablynx.com tel: fax: mobile: +32 (0)9 261 06 57 +32 (0)9 261 06 27 +32 (0)486 788 551 <https://www.plaxo.com/add_me?u=30065269879&v0=994426&k0=2009290972> Add me to your address book... <http://www.plaxo.com/signature> Want a signature like this? -----Original Message----- From: Xiao Qun Zhai [mailto:xqzhai@uclan.ac.uk] Sent: Wednesday, March 28, 2007 1:05 PM To: cyto-inbox Subject: conjugated anti mouse secondary ab Hello everyone, I am looking for PE or FITC conjugated anti mouse secondary ab from Goat or Horse. My primary antibodies are mouse monoclonal IgG1 and IgG2a class. I have tested F0479 (goat anti mouse-FITC) from DAKO, it works. But I need to get the strongest signal if possible. Does anyone know any good secondary ab? Thanks Sunny ----------------------------------------------------------------------- THIS E-MAIL MESSAGE IS INTENDED ONLY FOR THE USE OF THE INDIVIDUAL OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE. If the reader of this E-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately at ablynx@ablynx.com. Thank you for your co-operation. "NANOBODY" and "NANOCLONE" are registered trademarks of Ablynx N.V. -----------------------------------------------------------------------
