LSR II FACSFlow system

Hello all...

Please let me hear from you if you have an LSR II with the FACSFlow and HTS Systems.  My
questions are:

1) Would you purchase the FACSFlow System again?
2) Does the HTS use more sheath than regular tubes?

For those of you with the standard sheath system... How long does a tank last if running
samples all day long?

Thanks in advance!

Elizabeth

Elizabeth T. Kraus
Manager, Flow Cytometry core Facility
Baylor Institute for Immunology Research
3434 Live Oak, Suite 200.1
Dallas,  TX.  75204
214-820-3586
elizabkr@bhcs.com


-----Original Message-----
From: Julie Bertout [mailto:julie.bertout@ibl.fr]
Sent: Wednesday, March 28, 2007 8:58 AM
To: cyto-inbox
Subject: lymphocyte cell cycle analysis

Hello,

Does anybody have a protocol to study cell cycle of lymphocytes purified
from blood?

I suggested the regular PI staining I used for cell line
- fixation with cold EtOH 80%,
- storage at 4°C,
- stain 1 million cells with 100ug IP and 125ug RNase 30min @ 37°C
but the morphology of the cells completely changed after this treatment.
The lymphocyte were quiescent. The researcher did a positive control
using PHA to induce cell cycle but the populations changed even more...

Thank you for your advices.

Julie Bertout
cytometry lab
Institut Pasteur de Lille
1 rue du professeur Calmette
59800 Lille
France

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