cell line sorting on Aria

Hello,

We would like to sort cell line transfected with GFP in order to do a 
cloning but I had a hard time setting up the Aria...
No problem to sort lymphocytes or eosinophils from blood or spleen in 
high speed sorting but the medium and low sorting required for cell line 
sorting are difficult to set up.

Does anybody sort transfected cell line ? what conditions do you use : 
concentration of cells? events/s recommended? amplitude usually used? 
pressure (BD told me sometimes you have to play a little bit with it...)?

Thanks for your answers.

Julie Bertout
cytometry lab
Institut Pasteur de Lille
1 rue du professeur Calmette
59800 Lille
France