Dear Flowers, We are in the process of developing a clinical assay to characterize different T cell subsets. I need inputs/suggestions/comments on the following naming conventions according to the phenotype. Any help is very much appreciated. CD45+ Lymphocytes CD3+ T cells CD3+CD4+ Helper T cells CD3+CD8+ Cytotoxic T cells CD4+CD45RO-CD45RA+CD27+CD62L+ Total helper Naive T cells CD4+CD45RO+CD45RA- Total helper Memory T cells CD4+CD45RA-CD45RO+CD27-CD62L- Helper Effector Memory T cells CD4+CD45RA-CD45RO+CD27+CD62L+ Helper Central Memory T cells CD4+CD25+ Total TREGs CD4+CD28+HLA-DR+ Activated Helper T cells CD8+CD45RO-CD45RA+CD27+CD62L+ Total cytotoxic Naive T cells CD8+CD45RO+CD45RA- Total cytotoxic Memory T cells CD8+CD45RA-CD45RO+CD27-CD62L- cytotoxic Effector Memory T cells CD8+CD45RA-CD45RO+CD27+CD62L+ cytotoxic Central Memory T cells CD8+CD28+HLA-DR+ Activated cytotoxic T cells CD4+CD25+CD127loCD45RO+CD45RA- Natural TREGs CD4+CD25+CD62L+CD45RO+CD45RA- CD4+CD25+CD127loCD45RO-CD45RA+ Naïve TREGs CD4+CD25+CD62L+CD45RO-CD45RA+ CD4+CD25-CD127hi Tr1/Th3 These are just tentative names given to the subsets according to the phenotype and based on the replies, we may end up changing these. Thanks, ************************************************ Vijay Nandakumar Development Cellular and Molecular Immunology Lab. Mayo Clinic, Rochester, MN nandakumar.vijay@mayo.edu ************************************************* At the end of the day, just be Happy !!! :)
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