Hi Dongjuan Having obtained a small sample of DRAQ5 from Roy Bongaerts at the IFR last week I have just played a bit with the dye. As Howard said, signal is poor, but does exist. On the Elite I excite with the HENE and measure with a 675nm band pass. I might improve things with a different filter (long pass to catch more signal) but my current configuration I get a much better signal to noise using ethidium bromide with the 488nm laser and a og630 (schott) longpass. Only for the EB staining I have to deenergize or block the pumps which does not seem to be necessary with the DRAQ5. Exciting DRAQ5 with the 488 does not yield enough signal. According to Advanced Analytical DRAQ5 gives a better s/n than Syto 62. They use a 639nm laser diode but I do not know their filter combination. However I can not confirm that by lack of Syto 62 to play with. When it comes to sorting DRAQ5 stained cells you might have a problem as the dye is said to be toxic, but again, I have not had time to investigate that. Good luck Gerhard > -----Original Message----- > From: Dongjuan DAI [mailto:daidj00@gmail.com] > Sent: 14 December 2006 15:34 > To: Cytometry Mailing List > Subject: DRAQ5 staining of bacterial cells > > > Hi Everyone, > > Have you ever used this dye, DRAQ5 from Biostatus, on > bacteria? I tried it on both E.coli K12 and Pseudomonas > aeruginosa. But I cannot get any bacterial cells stained. Do > you have any ideas why I fail? I tried from 1ug/ml to 20ug/ml > of DRAQ5. > > Thank you, > Dongjuan > > -- > Dongjuan Dai, Doctoral Student > Dept. of Environmental Health Sciences > School of Public Health > the University of Michigan, Ann Arbor > 109 Observatory St. 6645 SPH Tower, MI 48109 >Received on Tue Dec 19 11:38:00 2006
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