mouse stem cell protocol and Dynabeads

Dear all,

I recently read a protocol about using Dynabeads to
enrich mouse stem cell. The protocol comes with some
graphs. I think I might miss something here.  Based on
the protocol I can’t tell which sample the authors use
to run the FLOW: in order to get the pictures shown in
the protocol do they use pre-beads (before enrichment)
or post-beads sample(after enrichment). 1) If these
graphs come from pre-beads sample,  then how do the
pictures look like for those from post-beads samples?
2) If the graphs collect from post-beads samples it
looks to me  there are still about 90%	contamination
in the negative portion after enrichment.  What is
percentage of the Lin negative portion before the
enrichment? 3) I notice that the beads used in the
protocol are recommended to isolate macromolecules
such as nucleic acids and organelles only BUT  not the
one used for isolate cells. Are there any good reasons
to use these specific beads instead used the ones
recommended to isolate cells?  Does anyone there use
the same beads as the one in the protocol  to isolate
cells? What kind of purity do you get if you follow
the protocol?

Thank you very much for your time,


Li


1)This protocol can be found in 

Current Protocols in Immunology (2005)
22B.1.1-22B.1.13

2) Link to the protocol

https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.viewUnit&treeNodeID=9E6632E
10870F6877330C766D4C5BFE2&objectid=66759507FE3FF1C46FB9D13DD5C9616B


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