Coherent Sapphire laser and BD FACSVantageDiva

Hi all,

We've have just had a 200mW Coherent Sapphire installed on our	
VantageDiva to replace on old Coherent I90. So far we are very happy  
with it apart from one aspect - we can no longer see the beam spot on  
the stream. This makes alignment somewhat more difficult than  
previously. I think we are getting used to it but it would be nice to  
be able to see the spot.

Our Coherent engineer recalled hearing of this as a problem but  
couldn't remember any details. Our BD engineer had never seen it before.

Has anyone else seen this? Any suggestions for what might be behind  
it and how we might fix it?

Regards,

Adrian Smith
Centenary Institute, Sydney, Australia