Hello Flowers, I have been trying to set up Hoechst/ PyroninY combined with cell surface staining on a MoFlo, using the published protocol. My UV laser(50mw) is in 2nd position, HeNe in 3rd. I can see reasonable signals for each single color: Hoechst, PyroninY, Sca1-PECy7, Ckit-APC, and Lin-APC Cy7, and can set up reasonable compensations. However, when I look at the multicolor sample the pyronin signal drops to background (except on one occasion), while every other signal looks OK. Does anyone have any insights as to what might be happening? This does not happen when we run the sample on the LSRIII. Joanne Joanne Yetz-Aldape Manager-Flow Cytometry Core Facility Cutaneous Biology Research Center MGH-East; Building 149 13th Street Charlestown, MA 02129 Phone: 617-724-0247 FAX: 617-726-4453 E-mail: joanne.yetz-aldape@cbrc2.mgh.harvard.eduReceived on Tue Dec 12 14:18:00 2006
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