Hi eveyone, I am working with CD107
antibody as a marker of CTL degranulation, in combination with several other markers. I
am having problems with unstimulated controls, usually finding high values of CD8 cells
positive for CD107. Is there any way to solve this problem? Even if I simply substract
the unstimulated control from the stimulated one, I find unusually high values for
CD8+CD107+ peptide-specific cells. So I suspect this is an artifact rather than real
values.
Many thanks
José M Benito
Infectious Diseases
Hospital Carlos III
Madrid
Spain
