RE: GFP

PFA fixation dampens GFP signal, although fixation can be adjusted to still get some detection.  This has been discussed many times on this list; see www.cyto.purdue.edu/hmarchiv/index.htm

=======================================================
Rachel M. Gerstein, Ph.D.
Associate Professor
Department of Molecular Genetics and Microbiology
Graduate Program in Immunology/Virology
University of Massachusetts Medical School
55 Lake Avenue North
Worcester, MA 01655-0002
(508) 856-1044
(508) 856-5920 (FAX)


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> From: 	Ibtissam Abdul-Jabbar
> Sent: 	Thursday, December 7, 2006 10:31 PM
> To:	Cytometry Mailing List
> Subject:	FW: GFP
> 
> Dear Flowers would you please answer Ray on his question { whether detection of eGFP by FACS is enhanced or hindered by paraformaldehyde fixation of cells prior to cytometry?}
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>  
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> Many thanks
> 
> iaj
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> 
>   _____  
> 
> From: Ray Steptoe 
> Sent: Thursday, 7 December 2006 4:10 PM
> To: Ibtissam Abdul-Jabbar
> Subject: GFP
> 
>  
> 
> Hi Ibtissam,
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>  
> 
> Would it be possible to ask the flow users list whether detection of eGFP by FACS is enhanced or hindered by paraformaldehyde fixation of cells prior to cytometry?
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>  
> 
> Many thanks
> 
> Ray
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>  
> 
> -----------------------------------------------------------------
> 
> Raymond J. Steptoe  PhD
> 
> The Centre for Immunology & Cancer Research 
> 
> The University of Queensland 
> 
> Princess Alexandra Hospital 
> 
> Level 4, R Wing, Building 1
> 
> Woolloongabba,  QLD 4102 
> 
> AUSTRALIA
> 
> Telephone: + 61 7 3240 5393	Facsimile: + 61 7 3240 5946 
> 
> Internet: http://www.cicr.uq.edu.au 
> 
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