Re: BD HTS for LSRII/Canto

Adrian,

I use the HTS on the LSRII for propidium iodide staining of DNA for cell 
cycle analysis.  I recommend getting it!  It is easy to set up and use.  I 
haven't had any problems thus far besides occasional salt build-up on the 
front pump.  The only draw back with the HTS compared to the plate reader 
I used on the FACScan is that it has to be hooked up every time you want 
to use it (more than just attaching the tube to the SIT), but it is not 
hard and takes less than 30 seconds.  The only worry is that I might one 
day accidentally drop it.  One nice feature it has over the FACScan plate 
reader is that you can set the sample volume and speed for each individual 
well before you start the run.

The HTS has been extremely reliable.  It saves me a great deal of time and 
allows me to run many more samples than I normally would.  It cuts down on 
sample preparation time, and I don't have to sit in front of the LSRII for 
hours running one tube at a time.  My plan is to adapt some of the other 
work I'm doing to the HTS because I like it so much.  I definitely think 
it is worth buying!

Let me know if you have any other questions.

Kim Smitheman



"Adrian Smith" <a.smith@centenary.usyd.edu.au> 
07-Dec-2006 20:44
To
"Cytometry Mailing List" <cytometry@flowcyt.cyto.purdue.edu>
cc

Subject
BD HTS for LSRII/Canto






Hi all,

We are considering adding a 96-welll plate reader to LSRII or Canto 
and I'm putting together a grant application for the BD HTS.

I'm looking for feedback from current users on reliablity and ease of 
use.

I'm also particularly interested in what people are using it for? 
What sort of efficiency improvements does it provide over tubes in 
non-highthroughput scenario (ie most of what we do requires 
reasonably large files).

I know I want one - I just need some convincing arguments for the 
funding committee :)

Regards,

Adrian Smith
Centenary Institute, Sydney, Australia