RE: sodium azide: how to prepare a buffer from the powder?

From: Guy Hermans <Guy.Hermans@ablynx.com>
Date: Tue Sep 19 2006 - 04:47:52 EDT
Hi F.,
 
please re-read the instructions carefully - I'm sure it doesn't really say it's incompatible, because it is.
 
What is incompatible is (concentrated) sodium azide solutions and lead plumbing. A chemical reaction may occur between the azide and the metal, leading to explosive (not necessarily exploding) pipes	- lead azide is used as an explosive and I seem to recall reading that the explosive in car air bags is some azide as well.
 
See http://www.cdc.gov/Niosh/78127_13.html for a description of the problem which is relevant to our typical lab situation. Read http://www.ehs.neu.edu/naazide1.html for more info on sodium azide itself - it stipulates clearly the sodium salt is NOT explosive, and readily soluble in water.
 
Guy
 



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-----Original Message-----
From: florent crepineau [mailto:florentcrepineau@yahoo.fr] 
Sent: Monday, September 18, 2006 12:41 PM
To: cyto-inbox
Subject: sodium azide: how to prepare a buffer from the powder?


Dear all,
 
this is not strictly speaking a flow cytometry question but more of a chemistry question. I have purified antibodies on a column and I would like to store them in a buffer containing sodium azide. I ordered the powder but reading the MSDS, it seems impossible to use it as it is incompatible with water and explodes. How do you do to put sodium azide powder in a buffer solution?
Thank you in advance.
 
Best regards
 
Florent



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Received on Tue Sep 19 15:18:00 2006

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