Dear Petra and Joern - Here in our clinical flow cytometry lab, we use 10% bleach, followed by distilled water, for cleaning. We generally use one tube with 10% bleach, followed by 3 tubes of distilled H2O All are run for 200 seconds each. We also use 10% bleach and DH2O for vaccuum cleansing. It is not necessary to use undiluted bleach. We only use undiluted bleach for a large clog or other troubleshooting - it will affect your lines. The most important point is that the 10% bleach needs to be made fresh daily. We run T cell subsets on a large population of HIV positive patients. We use a lysing system that inactivates or kills the HIV virus. We also always observe standard precautions. You can also get cleaning agents that are specifically made for cleaning in potentially biohazardous areas (such as a laboratory). These were the instrument manufacturers' instructions. They have worked very well for us for about 15 years now. Indeed, better safe than sorry, although bleach stronger than 10% will not be any more effective. Hope this helps. Best regards - Leslie Leslie C. Badore, CLS(NCA) Immunology Charge Technologist Pathology & Laboratory Medicine, EP1-100 Fletcher Allen Health Care 111 Colchester Avenue Burlington, VT 05401 email: Leslie.Ryan@vtmednet.org phone: (802)847-5373 fax: (802)847-4103 Confidentiality Notice: This message, and any attachments, may contain information that is confidential, privileged, and/or protected from disclosure under state and federal laws that deal with the privacy and security of medical information. If you received this message in error or through inappropriate means, please reply to this message to notify the Sender that the message was received by you in error, and then permanently delete this message from all storage media, without forwarding or retaining a copy.Received on Tue Sep 19 13:58:00 2006
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