Ole-Kristian Hess Nilsen wrote: >I need advice regarding a used FACSScan (including old software). >It has not been used the last 6 months (worked well when in use, quite >new laser) due to buying a new Aria. >I want to count marine bacteria and maybe viruses (mainly by nucleic >acid dye). Could this be done with a FACSScan? Is my object feasible or >will it cause too much problems and money? The problem, if there is one, is with forward scatter, which won't give you big signals because the detector is a diode and not a PMT. Write to Penny Chisholm at MIT (chisholm@mit.edu), and ask her if someone in her lab can give you the details of how they modified their FACScan to increase scatter sensitivity. They've been measuring marine bacteria (including Prochlorococcus, which they discovered) for decades. You may also be OK triggering on side scatter for the bacteria. As far as the viruses go, you'll be triggering on nucleic acid dye fluorescence for those, and the FACScan should be fine. -HowardReceived on Mon Apr 3 11:58:00 2006
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