RE: human embryonic stem cells hs1,hs9

Hi Gene:

Here is a link to University of Virginia's policy for handling Human Cell
Lines: http://keats.admin.virginia.edu/bio/uva_human_cell_line_policy.cfm

As the policy states that all human cell lines must be handled as BSL-2 I
have included the handling requirements as defined here. As sorting
certainly involves the generation of aerosols I think containment should be
a must.

Biosafety Level 2 (BL2) is similar to Level 1 and is suitable for work
involving agents of moderate potential hazard to personnel and the
environment. It differs in that: (1) laboratory personnel have specific
training in handling pathogenic agents and are directed by competent [sic]
scientists; (2) access to the laboratory is limited when work is being
conducted; and (3) certain procedures wherein aerosols are created are
conducted in biological safety cabinets or other physical containment
equipment.

We use an aerosol containment hood on our FACSVantage for the sorting of all
human cell lines.

 

Joanne Lannigan, MS

Director, Flow Cytometry Core Facility

University of Virginia

Jordan Hall, Room 7067

P.O. Box 800734

Charlottesville, VA 22908-0734

Office: 434-924-0274

Lab: 434-243-2695

Fax: 434-982-1071

email: joannelannigan@virginia.edu

-----Original Message-----
From: Pizzo,Eugene [mailto:Pizzo@NSO1.UCHC.EDU] 
Sent: Wednesday, March 08, 2006 11:20 AM
To: cyto-inbox
Subject: human embryonic stem cells hs1,hs9

 

Folks,

 

A researcher here at UCONN is involved in starting up a Stem Cell Facility
here and would appreciate some feedback from the community concerning
conflicting information he has received about the necessity for containment
when sorting human 

embryonic stem cell lines, H1  and H9. What precautions are investigators
using with these cells in particular? Containment, no containment? You can
respond to this email or if you would prefer to contact Leonardo Aguila,
Ph.D directly his email address is

Aguila @nso1.uchc.edu

 

 

 

 

Thanks

 

Gene Pizzo

UCONN Health