Hi Claude, as far as I know there are no ready-made tandem dye kits available other than the Prozyme kit <http://www.prozyme.com/phycolink/index.html> http://www.prozyme.com/phycolink/index.html with Oyster dye rather than the more common Cy/Alexa acceptor dyes. They have PE and APC kits, so you may want to try APC or PE and pair that with the PE/Oyster dye tandem for the second channel. I haven't tried the tandem dye kit, but have had good results with their PE, APC and PerCP kits. Mario Roederer's website also lists PE tandem dye labeling procedures; check out www.drmr.com <http://www.drmr.com/> Best of luck, Guy Next generation therapeutic <http://www.ablynx.com/> antibodies Guy Hermans, PhD Project Leader Ablynx NV Technologiepark 4 B-9052 Zwijnaarde Belgium guy.hermans@ablynx.com tel: fax: mobile: +32 (0)9 261 06 57 +32 (0)9 261 06 27 +32 (0)486 788 551 <https://www.plaxo.com/add_me?u=30065269879&v0=994426&k0=2009290972> Add me to your address book... <http://www.plaxo.com/signature> Want a signature like this? -----Original Message----- From: Stéphanie Beq [mailto:sbeq@pasteur.fr] Sent: Friday, January 20, 2006 4:33 PM To: cyto-inbox Subject: Ab labelling APC PE-Cy7 or APC-Cy7 Dear flowers, A question from a collegue for you: I would like to label these two antibodies, anti-mouse Dtk (Tyro 3) obtained from R&D ref N° MAB 759, and anti-mouse Axl also from R&D ref ° MAB 8541. I ordered purified Abs, and would like to label them with either APC, or PE-Cy7, or APC-Cy7. Do you know a working "kit" or proccedure, knowing that anti-Dtk is limited in quantity (500 microgr) and anti-Axl is also limited (100 microgr). Thanks, Claude Roth -- Claude Roth Laboratoire Immunité Cellulaire Antivirale Departement Immunologie Institut Pasteur 28 rue du Dr Roux 75724 Paris Cedex 15 France Tel: 33-1-40 61 32 71 33-1-45 68 88 64 Fax: 33-1-45 68 89 42 e-mail: clroth@pasteur.fr -------------------------------------------------------------- Beq Stéphanie PhD Unité des Virus Lents Département de Virologie Institut Pasteur 25 rue du Docteur roux 75724 cedex 15 Paris FRANCE tel: +33 1 40 61 32 24 Fax +33 1 40 61 31 67 ____________________________________ ----------------------------------------------------------------------- THIS E-MAIL MESSAGE IS INTENDED ONLY FOR THE USE OF THE INDIVIDUAL OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE. If the reader of this E-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately at ablynx@ablynx.com. Thank you for your co-operation. "NANOBODY" and "NANOCLONE" are registered trademarks of Ablynx N.V. -----------------------------------------------------------------------
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