Dear FLOWERs, I am now posting a question not related to flow cytometry. But I have found the list very helpful and coming up with ideas in other areas unrelated to flow cytometry too. My questions are related to storage of sera in serum bank. I know many of you may be maintaining sera collection for diagnostic assays (ELISA). 1. What is the size of the aliquots to be stored? For ELISA we need only very small volumes. But if we store small volumes, even at -80C there is slow evaporation and the sera become almost solid. If you aliquot 1 ml etc, you may have to repeatedly freeze-thaw the sample. 2. Can we store the sera in glycerol as 1:1 mixture at -20C, so that the sera will not freeze? 3. What type of cryovials are best suited, without evaporation? 4. How long are the sera stable and usable at +4C? -20C? -80C? Liquid nitrogen? 5. Are there any studies on how the duration of storage affects the antibody titre? My Happy Christmas & New Year wishes to you and your family. Thanking you in advance for sharing your experience, Sincerely, Alamelu Raja --------------------------------- Yahoo! Shopping Find Great Deals on Holiday Gifts at Yahoo! ShoppingReceived on Tue Dec 27 13:38:00 2005
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