We have been experiencing compensation problems with the APC and PE Cy7 tandems. Often the spillover to the donor channel(PE/APC) is greater than the signal intensity in the Cy7 detector. I am not sure what is the cause, light exposure, temperature or some other source. This is happening to several customers and as far as I know the Ab supply is fine( as checked out by capture beads). It appears to be occurring during the sample processing stage. If these compounds are extremely sensitive in handling, I would expect this more frequently than currently seen. I was planning on checking this phenomenon using some capture beads and various temperature and/or light conditions. If anyone has pursued this further please contact me.Thanks for your help. Paul E. Champoux Research Services Coordinator Center for Immunology University of Minnesota 612 626-3068 612 625-2199(Fax) www.immunology.umn.eduReceived on Fri Dec 23 19:58:00 2005
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