RE[2]: intracellular staining of serotonin

There is a reference for a flow method in platelets (Br J Hematol v116,
604-611 (2002). In addition, there is a large body of work on
formalin-induced serotonin fluorescence mostly in neural tissues. Depending
on the specific question, one of these methods may provide the answer you
need. 

-Jim Weaver

-----Original Message-----
From: Prussin, Calman (NIH/NIAID) [E] [mailto:CPRUSSIN@niaid.nih.gov] 
Sent: Monday, December 19, 2005 5:58 PM
To: cyto-inbox
Subject: RE: intracellular staining of serotonin


Stacy,

I don't think this is technically possible using present day technology. All
of the fix and perm protocols rely on fixation to immobilize the (usually
protein) antigen. Serotonin is a small molecule (176 Daltons) and thus would
be unlikely to be immobilized by current fixation techniques, which
typically rely either on cross-linking or denaturing proteins to immobilize.
As soon as the cells are permeabilized, the serotonin would leak out.

Sorry.....

Calman
> _______________________
> Calman Prussin
> Laboratory of Allergic Diseases
> National Institute of Allergy and Infectious Diseases/ NIH
> 
> ----------
> From: 	Powell, Stacy
> Sent: 	Friday, December 16, 2005 12:45 PM
> To:	Cytometry Mailing List
> Subject:	intracellular staining of serotonin
> 
> Hello all!
> 
>  
> 
> Does anyone have any experience doing intracellular staining for 
> analyzing serotonin by
flow? If so, what method of permeabilization do you use?
> 
>  
> 
> Thanks!
> 
>  
> 
> Stacy Powell
> 
> Biologist
> 
> Southern Research Institute
> 
> 2000 Ninth Avenue South
> 
> Birmingham, AL  35205
> 
> 205-581-2243
> 
> powell@sri.org
> 
>  
> 
>