Stacy, I don't think this is technically possible using present day technology. All of the fix and perm protocols rely on fixation to immobilize the (usually protein) antigen. Serotonin is a small molecule (176 Daltons) and thus would be unlikely to be immobilized by current fixation techniques, which typically rely either on cross-linking or denaturing proteins to immobilize. As soon as the cells are permeabilized, the serotonin would leak out. Sorry..... Calman > _______________________ > Calman Prussin > Laboratory of Allergic Diseases > National Institute of Allergy and Infectious Diseases/ NIH > > ---------- > From: Powell, Stacy > Sent: Friday, December 16, 2005 12:45 PM > To: Cytometry Mailing List > Subject: intracellular staining of serotonin > > Hello all! > > > > Does anyone have any experience doing intracellular staining for analyzing serotonin by flow? If so, what method of permeabilization do you use? > > > > Thanks! > > > > Stacy Powell > > Biologist > > Southern Research Institute > > 2000 Ninth Avenue South > > Birmingham, AL 35205 > > 205-581-2243 > > powell@sri.org > > > >Received on Tue Dec 20 12:38:00 2005
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