Simona First, check that you have full vacuum to the machine from the wall. The house vacuum lines corrode, due to the bleach vapors from your waste tank. I've also found that the plastic vacuum lines in the machine can become clogged over time. You can try opening the control panel and use clamps to close the line from the stream waste collector to get more vacuum to the nozzle. I had to flush (several times!) the whole pathway from the waste collector to the tank with detergent. I forced detergent BOTH ways to flush the tubing, and I saw that it was indeed quite full of junk. The little fittings can become clogged easily. At 05:38 AM 12/16/2005, you wrote: >Hi all, >I have a problem with Fill and nozzle flush on Facs Vantage SE + turbo >optional >I work at 20 PSI and when the nozzle is clogged I put on fill and I push >the bottom nozzle flush, but the stream go to back very slowly and is not >enough to clean the nozzle. >This happen also when the nozzle is not clogged!! > >Any comments are welcome >Many thanks in advance > >Simona > > >-- >Simona Ronzoni >Flow Cytometry and Imaging Core >Experimental Oncology Department >European Institute of Oncology >via Ripamonti, 435 20141 Milan Italy >Phone: ++39-02-57489880 Fax: ++39-02-57489851 >e-mail: simona.ronzoni@ifom-ieo-campus.it >http://www.ifom-ieo-campus.it >http://imaging.altervista.org ---Dennis Dennis J. Young Flow Cytometry Shared Resource Moores UCSD Cancer Center Room 2314 Bay 2Q 3855 Health Sciences Drive #0803 La Jolla, CA 92093-0803 Mail:<<mailto:djyoung@ucsd.edu>> WWW:<<http://cancer.ucsd.edu/flow/>> Telephone:(858) 822-0407 FAX: (858) 822-0403Received on Mon Dec 19 14:18:00 2005
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