Hello All, Thank you for the helpful responses. Here is a brief summary of the responses for those who are interested. A discrepancy between CFU and cytometric counts of bacteria is commonly observed. Cytometric counts are often greater than numbers of live cells determined by traditional plating assays. This is due to a variety of possible factors: - enumeration by flow may allow counting non-bacterial events (noise); all buffers must be filtered to prevent this - staining methods based on membrane integrity do not indicate reproductive capacity - plating efficiency is not 100% - precision and accuracy of counting bead method is not 100% Sincerely. Terry McGinn Therese M. McGinn Ph.D. Senior Research Scientist Benchmark Biolabs, Inc. Phone: 402-475-8104 Fax: 402-475-8511 www.benchmarkbiolabs.com ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager. This footnote also confirms that this email message has been swept by MIMEsweeper for the presence of computer viruses. www.mimesweeper.com **********************************************************************Received on Thu Dec 15 13:18:00 2005
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