hi Jane how was the GFP introduced ? transient transfection or stable expression from a retrovirus ? we - and many others - have done this repeatedly, without any problem. do they have both positive and negative controls that have been analyzed in parallel ? thanks, Rachel ======================================================= Rachel M. Gerstein, Ph.D. Associate Professor Department of Molecular Genetics and Microbiology Graduate Program in Immunology/Virology University of Massachusetts Medical School 55 Lake Avenue North Worcester, MA 01655-0002 (508) 856-1044 (508) 856-5920 (FAX) > ---------- > From: Jane S. Miller > Sent: Tuesday, December 13, 2005 4:50 PM > To: Cytometry Mailing List > Subject: Sorting GFP-labeled fibroblasts to culture > > Dear Flowers, I have a client who is trying to sort GFP-labeled > fibroblasts to grow in culture. The cells are sorted in PBS into > culture medium, using a FACSCaliber. After spinning down, GFP- labeled > cells were observed under the fluorescent microscope. They were plated > and looked at the next day under the fluorescent microscope again. The > cells that had attached were not fluorescent. Any ideas about what is > happening; are the labeled cells not viable or not producing GFP now? > Thanks for any help you can offer. Jane Miller, Dept. Micro. Mole. > Path., TX A&M Hlth. Sci. Ctr., College Station, TX 77843 > >Received on Thu Dec 15 12:38:00 2005
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