Dear Flowers, I have a client who is trying to sort GFP-labeled fibroblasts to grow in culture. The cells are sorted in PBS into culture medium, using a FACSCaliber. After spinning down, GFP- labeled cells were observed under the fluorescent microscope. They were plated and looked at the next day under the fluorescent microscope again. The cells that had attached were not fluorescent. Any ideas about what is happening; are the labeled cells not viable or not producing GFP now? Thanks for any help you can offer. Jane Miller, Dept. Micro. Mole. Path., TX A&M Hlth. Sci. Ctr., College Station, TX 77843Received on Wed Dec 14 10:38:00 2005
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