RE: flow cytometer choice for bacteria

Hello,

I have used both the EPICS and the FACS Calibur for some pretty creative
bacteria work. Both work fine. Pick your flavor!


Caroline 


-----Original Message-----
From: Larry Arnold [mailto:lwarma@med.unc.edu] 
Sent: Friday, December 09, 2005 1:57 PM
To: cyto-inbox
Subject: Re: flow cytometer choice for bacteria


William

We do quite a bit of sorting of various bacteria on our MoFlo.	It 
works very well.  We trigger on log SSC.  WE have never found it 
necessary to use a fluorescence trigger as we can always resolve the 
bacteria by scatter.

Larry

At 10:22 AM 12/8/2005, you wrote:
>Dear Flowers,
>I was wondering if anyone could give me advice on the suitability of
>the various high
>speed sorters (Mo Flo, BD Aria, Epics Altra etc) for analysis and 
>sorting bacteria. Most
>people, the manufacturers reps included, are obviously more used to 
>eukaryotic cell work.
>
>Many thanks,
>William Gaze

Larry W. Arnold, Ph.D.
Research Professor and Director, Flow Cytometry Facility Department of
Microbiology and Immunology CB# 7290 University of North Carolina Chapel
Hill, NC 27599
Phone: 919-966-1530
FAX: 919-962-8103