Re: GFP labelled virus infectivity by FACS

Hi Benjamin, please check these papers that my lab has published on this
topic. Please let me know if you need any help with the protocols,
1:	Nunez R, Fraefel C, Suter M, Nunez-Liman A, Liou HC, Ackerman M.
	Assessment of a cellular vaccination approach consisting of crawling
dendritic cells (CDCs) transduced with HSV-1-Deltapac vectors.
Immunol Res. 2004;30(1):105-24.

2:	Schwyzer M, Fischer-Bracher C, Fraefel C, Bachi T, Nunez R, Engels M,
Ackermann M.	Transduction of Vero cells and bovine monocytes with a
herpes simplex virus-1 based amplicon carrying the gene for the bovine
herpesvirus-1 Circ protein.
Vet Microbiol. 2002 Apr 22;86(1-2):165-74.

3:	Nunez R, Ackermann M, Saeki Y, Chiocca A, Fraefel C.
Flow cytometric assessment of transduction efficiency and cytotoxicity of
herpes simplex virus type 1-based amplicon vectors.
Cytometry. 2001 Jun 1;44(2):93-9.
Regards Rafael

On Thu, December 8, 2005 3:03 pm, Galen, Benjamin said:
> Hi,
>
> I would like to monitor GFP-tagged HSV infection of cells using FACS.  I
> am wondering if anyone can suggest relevant references or has any advice.
> Will FACS be sensitive enough to detect a small number of infected cells?
>
> Thanks,
>
> Benjamin Galen
> Pediatric Infectious Diseases
> Mount Sinai School of Medicine
>


Rafael Nunez MD.
Assistant Professor of Medicine and Pathology.
University of Illinois at Chicago.
Director Cancer Center Flow Cytometry Core Facility
Room 3211 MBRB, M/C 837
900 S. Ashland ave, Chicago, Il 60607
Phone 3123550123