CaspaTag

Hi Everybody

We're having a hard time interpreting our caspatag binding data on
peripheral blood.  We're looking a lymph's, mono's, grain's and blasts,
in AML peripheral blood post drug treatment and assessing apoptosis,
necrosis using a four color cocktail of CD45, caspatag, annexin V and
7AAD.  Our caspatag binding is uniformly weak (it's never bright
expression) but positive on all monocytes from our untreated to our drug

treated specimens. In contrast, a defined negative and positive
population is found in granulocytes.  Lymphocytes and blasts are
generally negative of any caspatag binding.  Is the CaspaTag binding on
monocytes real or is this non specific binding?

All our specimens are run unstained, single marker, fluorescence minus
one, and finally all together.	We try to steer clear of istotypes....

Thanks in advance for your thoughts,
Rakesh

Rakesh Nayyar
Cytoquest Corp.