Re: flow cytometer choice for bacteria

From: Larry Arnold <lwarma@med.unc.edu>
Date: Fri Dec 09 2005 - 14:57:08 EST
William

We do quite a bit of sorting of various bacteria on our MoFlo.	It 
works very well.  We trigger on log SSC.  WE have never found it 
necessary to use a fluorescence trigger as we can always resolve the 
bacteria by scatter.

Larry

At 10:22 AM 12/8/2005, you wrote:
>Dear Flowers,
>I was wondering if anyone could give me advice on the suitability of 
>the various high
>speed sorters (Mo Flo, BD Aria, Epics Altra etc) for analysis and 
>sorting bacteria. Most
>people, the manufacturers reps included, are obviously more used to 
>eukaryotic cell work.
>
>Many thanks,
>William Gaze

Larry W. Arnold, Ph.D.
Research Professor and Director, Flow Cytometry Facility
Department of Microbiology and Immunology
CB# 7290
University of North Carolina
Chapel Hill, NC 27599
Phone: 919-966-1530
FAX: 919-962-8103 
Received on Mon Dec 12 13:18:00 2005

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