S-Phase marker

Hello Flow community,

I have a phd student with following question.

Thanx in advance

Steffen

Here is the question:

Hello Flowers,

I’m a PhD-Student at the Institute of Toxicology at university in 
Mainz, germany.

I have a short question:
I work with human cancer cell lines by treating with DNA-damage agents. 
Before the cells undergo Apoptosis I observe a late S-Phase or a 
G2/M-Phase cell cycle block by Propidium Iodide (PI) Staining.
Now I’d like to discriminate the S-Phase and G2 or M-Phase using a PI 
Staining combined with a specific S-Phase marker (perhaps a specific 
S-Phase protein).
Has anybody an idea witch marker is useful for this kind of 
discrimination?

Thanks very much   Steffen Naumann


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Dr. Steffen Schmitt
FACS und Array Core Facility
NMFZ- Johannes Gutenberg Universität
Obere Zahlbacherstr. 67
55131 Mainz

Tel.: +49 6131 39 30219
Fax: +49 6131 230506

www.facslab.toxikologie.uni-mainz.de
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