Hello, I was wondering if those of you who have done 3 colors off the red laser could share a little of your experiences. 1.Best combination: In the literature I've seen APC, APC-Cy5.5, APC Alexa 680, APC alexa 700, Alexa 700 APC Alexa 750, APC-Cy7. As far as commercially available reagents are concerned I would probably end up using APC, and APC-Cy7 together. What's the best middle fluorochrome with regards to brightness, and compensation. 2. What about Filter sets? Its seems pretty easy to get custom filters these days so how extensively have you compared different filter combos. Seems like a common filter for the middle fluorochrome is 700/20. Seems like you could go a little higher and wider but don't know if you run into comp issues with that. I know the spectra viewer is great for this kind of question but some real life experiences might tell a slightly different story. (then again maybe not) Any and all info is welcome. Thanks in advance. JamesReceived on Wed Dec 7 12:58:00 2005
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