Re: anybody ever sort out ...

From: Larry Arnold <lwarma@med.unc.edu>
Date: Wed Dec 07 2005 - 08:54:17 EST
Chris

We are getting ready to do something similar on our MoFlo.  We 
already sort some small bacteria with no problem (SSC log 
trigger).  We looked at polystyrene beads recently and were easily 
able to resolve beads down to 0.21um diameter.	If the mito stain 
bright enough you could also trigger on them.

Larry

At 04:23 PM 12/5/2005, you wrote:
>mitochondria?
>
>
>I've got somebody that wants to isolate mitochondria so they can 
>extract proteins.
>
>She says she can stain them.  But I'm just wondering about their 
>size, relative to the
>debris we'll have.
>
>thanks,
>
>Chris

Larry W. Arnold, Ph.D.
Research Professor and Director, Flow Cytometry Facility
Department of Microbiology and Immunology
CB# 7290
University of North Carolina
Chapel Hill, NC 27599
Phone: 919-966-1530
FAX: 919-962-8103 
Received on Wed Dec 7 12:38:00 2005

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