Robin, You might try putting some lube around the orange o-ring inside the sample station and a little on the black tube holder stage below, being careful not to get any on the sample tubing. It's easier to do this when the instrument is off, when the sample backflush catcher is pulled back. This problem usually occurs when it can't pressurize the sample tube. Good Luck. Kristie Kristie M. Gordon Flow Cytometry Core Facility Department of Medicine Columbia University College of Physicians & Surgeons 1130 St. Nicholas Avenue Rm938 New York, New York 10032 212-851-4574 kmg2109@columbia.edu ----- Original Message ----- From: Stingley, Robin L To: Cytometry Mailing List Sent: Wednesday, November 30, 2005 6:56 PM Subject: Aria stream shutdown w/load sample Dear flow experts, I've been having an intermittent problem with the stream shutting off when I load Accudrop beads, and it's getting worse. We've tried changing all sample lines and I've been backflushing before loading tubes, and have turned the flow rate down to 1.0. At first I thought that it was a problem with the first loaded tube of the day, but it has now happened after other tubes have loaded fine. It usually happens with the accudrop beads, and usually right before a sort customer walks in the door. It has happened even after I filtered the beads. It actually started a while ago with customer samples, but I assumed that they had clumped even though they were in filter-top tubes and I knew the customer(s) had filtered them. The error message says that the system detected a clog but if I immediately restart the stream, there is no problem and I can usually load the same tube of beads (or cells) and continue. My engineer is asking around to see if any other engineers have seen this, but I thought I'd check with this group. Any suggestions would be greatly appreciated. Thanks, Robin Robin Stingley, Ph.D. Flow Cytometry Core Facility Department of Microbiology and Immunology University of Arkansas for Medical Sciences Slot 511, Rm B504 4301 West Markham Street Little Rock, Arkansas 72205 Phone: 501-686-6927 E-mail: StingleyRobinL@uams.edu http://www.uams.edu/flowcytometry/ ================================================================================================ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ================================================================================================Received on Fri Dec 2 15:38:00 2005
This archive was generated by hypermail 2.1.8 : Sat Jan 14 2006 - 22:04:00 EST
