Hello Flowers, Need some help for loss of sensitivity in red range of detection on a MoFlo. We are using a HeNe laser model 127 from Spectra-Physics. After many troubleshooting calls and hands-on work (switching PMTs for new ones, realignment, change of pinhole for detection, and many other things...) there seems to be a loss of sensitivity in the dimmer populations. This happens in both channels or detectors ( red sensitive PMTs) dedicated for the red laser. Even though we managed to measure the output of the laser (34mW) is there something I can try before calling a laser service guy? By the way, the laser has been purchased in early 90's and previously installed on a FACStar plus. Is it possible that the laser output or beam geometry is going off slowly? Any ideas? Eric Massicotte Responsable, Service de cytométrie en flux Institut de Recherches Cliniques de Montréal (IRCM) 110 ave. des Pins Ouest Montréal, Québec Canada, H2W 1R7 Téléphone: (514) 987-5724 Télécopieur: (514) 987-5736Received on Wed Nov 16 15:38:00 2005
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