Enid and other flow cytometry users. I believe daily measurements should be made using alignment beads in linear. Preliminary data suggest the larger beads are superior than smaller beads. However all beads larger than 2.5u can be used under proper handling conditions. I also believe that sensitivity measurements should be made daily to insure the machine is clean and not contaminated. This is my opinion and not that of the ISAC organization. ISAC is currently in the process of discussing and releasing guidelines for maintaining, aligning and calibrating flow cytometer. This topic will be discussed in detail at a workshop on flow cytometry standards in ISAC 2006 (Quebec City May 20-24) . Naturally, ISAC does not support or endorse any of the companies products, but only an approach that should be used to QA a flow cytometer. Best wishes Bob Zucker Secretary of ISAC 2004-2008Received on Tue Nov 15 12:18:00 2005
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