Hi Mark, not stupid at all - but have a look at BD or Mol Probes' spectra viewers (URLs below) and you'll see PE has a broad excitation range with two peaks: the highest of which is actually ~532nm. This is why PE works like a charm on 532nm diode laser based instruments like the FACSarray. Guy http://www.bdbiosciences.com/spectra/ http://probes.invitrogen.com/resources/spectraviewer/ Next generation therapeutic <http://www.ablynx.com/> antibodies Guy Hermans, PhD Project Leader Ablynx NV Technologiepark 4 B-9052 Zwijnaarde Belgium guy.hermans@ablynx.com tel: fax: mobile: +32 (0)9 261 06 57 +32 (0)9 261 06 27 +32 (0)486 788 551 <https://www.plaxo.com/add_me?u=30065269879&v0=994426&k0=2009290972> Add me to your address book... <http://www.plaxo.com/signature> Want a signature like this? -----Original Message----- From: Mark D'Amico [mailto:MDAmico@panaceapharma.com] Sent: Friday, November 04, 2005 9:16 PM To: cyto-inbox Subject: excitation and emission Howdy, I have a simple, if not outright stupid question. I have a 532nm laser for excitation. I am using R-PE as my marker. The excitation peak is at 488nm. Is my laser wavelength too long? Mark D'Amico, Ph.D. Scientist Panacea Pharmaceuticals 207 Perry Parkway #2 Gaithersburg, MD 20877 (240) 243-8000 Ext. 206 mdamico@panaceapharma.com ----------------------------------------------------------------------- THIS E-MAIL MESSAGE IS INTENDED ONLY FOR THE USE OF THE INDIVIDUAL OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE. If the reader of this E-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately at ablynx@ablynx.com. Thank you for your co-operation. -----------------------------------------------------------------------
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