analyzing 3t3 fibroblasts

Hello all,

I was given some CD40L-transfected 3T3 fibroblasts and I'd like to make sure
they are indeed expressing CD40L.  Since our lab usually deals with blood
samples, should the staining protocol for the fibroblasts be different than
for PBMC? If so, what are some things I should consider to get the most
representative data of my cells on the FACsort?  Any recommendations would
be appreciated. Thanks

Bertha Cheng
UCSF
Immunogenetics and Transplantation