Does anyone have any experience with molecular probes Syto Red dyes for identification of blood or cultured human cells? I have tried DRAQ5 for this - and although it looks promising, I lose 2 channels out of 4 since it appears in FL3 on 488 excitation and also in FL4 on 633 excitation in our FacsCalibur system. It does not appear to influence FL1 or FL2 (FITC or PE) to any significant extent, so in that respect it is OK. However, I believe it is possible to use a Syto Red with 633 excitation for channel FL4 detection while retaining 488/FL3 for immunostaining, as well as FL1 and FL2. Can anyone confirm, and if so what in their experience is the best Syto Red dye to use? Robin Barclay Dr G Robin Barclay PhD Lead Scientist SNBTS Adult Cell Therapy Group SNBTS R&D Division Edinburgh, Scotland, UK (Honorary Senior Lecturer, University of Edinburgh)Received on Tue Nov 1 14:38:00 2005
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