FCS files and software compatability

From: Mr Simon Corrie <s369338@student.uq.edu.au>
Date: Sun Oct 30 2005 - 12:18:21 EST
Hi folks

Im doing a short research project in Seattle and as such will have 
data from three different machines (Moflo, LSR2, Beckman MPLFC500) and 
three different software (mainly analysis) packages (FACSDiva, Summit, 
CPX).

I am finding it difficult to analyse my data across softwares - 
generally the problem seems to relate to the high resolution of the 
FACSDiva package, such that opening data in summit or CPX gives me 
data in the very low quadrant (log) areas. To solve the problem I 
would like to find a convenient method to convert my FCS files from 
various sources into .txt files so that I can use Matlab or R to 
analyse the data.

So finally my questions are:
1) Can someone suggest a program for the batchwise conversion of FCS 
to text? (I have tried ldata, but the FCS2.0 and 3.0 files do not 
convert)

2) Can someone give me a reference to help me learn how to set up my 
statistical analysis in R? ie maybe the most recent guide to rflowcyt 
if thats what people are using or an easier method? (I can code in 
matlab.....R frankly looks scary with a lack of reference material..)

Any ideas would be much appreciated

Regards

Simon Corrie
PhD Student
Nanotechnology and Biomaterials Centre
University of Queensland
St Lucia 4072 QLD Australia
Received on Mon Oct 31 13:18:00 2005

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