Hi folks Im doing a short research project in Seattle and as such will have data from three different machines (Moflo, LSR2, Beckman MPLFC500) and three different software (mainly analysis) packages (FACSDiva, Summit, CPX). I am finding it difficult to analyse my data across softwares - generally the problem seems to relate to the high resolution of the FACSDiva package, such that opening data in summit or CPX gives me data in the very low quadrant (log) areas. To solve the problem I would like to find a convenient method to convert my FCS files from various sources into .txt files so that I can use Matlab or R to analyse the data. So finally my questions are: 1) Can someone suggest a program for the batchwise conversion of FCS to text? (I have tried ldata, but the FCS2.0 and 3.0 files do not convert) 2) Can someone give me a reference to help me learn how to set up my statistical analysis in R? ie maybe the most recent guide to rflowcyt if thats what people are using or an easier method? (I can code in matlab.....R frankly looks scary with a lack of reference material..) Any ideas would be much appreciated Regards Simon Corrie PhD Student Nanotechnology and Biomaterials Centre University of Queensland St Lucia 4072 QLD AustraliaReceived on Mon Oct 31 13:18:00 2005
This archive was generated by hypermail 2.1.8 : Sat Jan 14 2006 - 22:03:57 EST