Reducing background when staining with 1st and 2nd antibodies

From: Elkabetz, Yechiel/Sloan-Kettering Institute <elkabety@mskcc.org>
Date: Fri Oct 28 2005 - 15:32:54 EST
Hi,

I am staining human cells with a mouse IgM as 1st antibody and then
FITC-anti-mouse IgM as a 2nd. What can I do to reduce background? This
is because I get a non specific staining of the secondary, which makes
the negative population shifted to be very close to the positive. Maybe
an FC block?
Thanks

Techie
Received on Mon Oct 31 11:58:00 2005

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