FoxP3/CD4 vs FoxP3/CD25

Dear Ramunas,
All Treg cells have to have CD4 and CD25.   CD25 can be expressed AFTER stimulation of
CD4 cells, and it is only the cells which are CD4+CD25+ at rest, which are called Treg
cells.	 The only problem is that some T cells are in a state of 'stimulation' even at
rest, so it is possible to still overestimate Treg cells by measuring CD4+CD25+ cells.
Hope this helps.
Hemali
MD Student
Warwick University

Ramunas wrote:
Dear clinical FCM users,
i'd like to address several questions to your global
audience concerning Treg(FoxP3) staining:
1. is there the most preferable combination for
choosing between bivariate FCM profile of human
FoxP3/CD4 and FoxP3/CD25 cells? Which combination is
better? I mean, why should i use both CD4 and CD25 if
it's possible to choose only one of them (this is
clinical setting, not scientific - it's a matter of
cost efficiency);
2.what clone is preferable to use? PCH101?

thank you in advance!



Ramunas Janavicius
Institute of Immunology,
Vilnius University
Vilnius, Lithuania