Re: UV laser for BD FACS Vantage

From: William Telford <telfordw@mail.nih.gov>
Date: Mon Oct 10 2005 - 22:16:27 EST
Hello Leonid...

If you have the funds, the Spectra-Physics Vanguard 355 nm 350 mW Nd:YV04 
UV laser is working out very well on our FACSVantage DiVa.  No chilled 
water required, only 110V 20A electrical service required, and a 20,000 
hour anticipated lifetime.  Power and pointing stability are excellent.  We 
use about 150 mW of the total beam power (attentuated with a beamsplitter) 
and find the resulting performance indistinguishable from our previous 
krypton-ion laser.  Although more expensive than an ion, we expect it to be 
cheaper in the long run, with lower infrastructure and maintenance costs.

I believe that Lightwave Electronics is also working on a more powerful 
version of their 20+ mW XCyte Nd:YV04 laser for stream-in-air sorters, as well.

I have no commercial interest in either SP or LE.

Bill Telford
NCI-NIH

At 03:12 PM 10/7/2005, Leonid Volkov wrote:

>Dear colleagues,
>
>I am looking for the best (quality / price / durability) UV laser for BD 
>FACS Vantage. We need to excite DAPI or Hoechst and the UV line in the 
>Enterprise-laser is dead.
>
>Any help would be greatly appreciated.
>
>Thank you in advance.
>
>With best regards
>
>L. VOLKOV , Ph. D; M. D.
>
>Asst. en Cytometrie -Microscopie
>
>loc. 4867
>
>Service commun de cytométrie et de microscopie
>
>Centre de recherches cliniques
>
>3001, 12 av.Nord SHERBROOKE
>
>Quebec, CANADA
>
>		  J1H 5N4
>
>	  Tel: (819) 346-1110 ext 1-4867
>
>	  Fax: (819) 564-5215
>
>Leonid Volkov <leonid.volkov@chus.qc.ca>
Received on Tue Oct 11 16:58:00 2005

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