Hello Leonid... If you have the funds, the Spectra-Physics Vanguard 355 nm 350 mW Nd:YV04 UV laser is working out very well on our FACSVantage DiVa. No chilled water required, only 110V 20A electrical service required, and a 20,000 hour anticipated lifetime. Power and pointing stability are excellent. We use about 150 mW of the total beam power (attentuated with a beamsplitter) and find the resulting performance indistinguishable from our previous krypton-ion laser. Although more expensive than an ion, we expect it to be cheaper in the long run, with lower infrastructure and maintenance costs. I believe that Lightwave Electronics is also working on a more powerful version of their 20+ mW XCyte Nd:YV04 laser for stream-in-air sorters, as well. I have no commercial interest in either SP or LE. Bill Telford NCI-NIH At 03:12 PM 10/7/2005, Leonid Volkov wrote: >Dear colleagues, > >I am looking for the best (quality / price / durability) UV laser for BD >FACS Vantage. We need to excite DAPI or Hoechst and the UV line in the >Enterprise-laser is dead. > >Any help would be greatly appreciated. > >Thank you in advance. > >With best regards > >L. VOLKOV , Ph. D; M. D. > >Asst. en Cytometrie -Microscopie > >loc. 4867 > >Service commun de cytométrie et de microscopie > >Centre de recherches cliniques > >3001, 12 av.Nord SHERBROOKE > >Quebec, CANADA > > J1H 5N4 > > Tel: (819) 346-1110 ext 1-4867 > > Fax: (819) 564-5215 > >Leonid Volkov <leonid.volkov@chus.qc.ca>Received on Tue Oct 11 16:58:00 2005
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