Hi All, Just wondered if there is a consensus on whether cell surface staining (or intracellular staining) is better at 4 deg C (on ice) or room temperature. I've done all of my staining at 4 deg (per recommendation of the Ab manufacturer) with good results but I've scanned the recent literature and it seems that all methods papers I've looked at stains at RT. Looking forward to your comments before amending my procedure! Maloy Mangada, PhD Scientist, Cell Biology Acambis, Inc., 38 Sidney St., Cambridge, MA 02139 Email: maloy.mangada@acambis.com Phone: (617) 761-4288 FAX: (617) 494-1741 -------------------------------------------------------- This e-mail is intended for the exclusive use of the addressee(s) and may contain information that is confidential, privileged, and exempt from disclosure under applicable law. If you are not the intended recipient, be advised you have received this message in error and that any use, dissemination, forwarding, printing, or copying is strictly prohibited. If you received this email by mistake, please notify the sender immediately and destroy all copies of this message and any attachments. Please visit the website at www.acambis.comReceived on Thu Sep 29 14:38:00 2005
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