Maris: Carefully. We are handling live lentivirus infected cells as a RG-2 organism i.e. biosafety device for the sorter, separate facility, monitoring program, controlled air flow within the facility, disinfection after running one of these samples, gloves, gowns and masks for the operator etc. I did a search of the literature recently and it is not clear on how much of a risk lentivirus poses. Also, our local experts can't seem to agree amongst ourselves as to how much a risk one of these organisms creates. However I would rather do overkill and sleep well at night rather than take any risks. We are basically following the general guidelines given at the CDC web site for a BL2 facility. Nick >>> "Handley, Maris" <Maris_Handley@dfci.harvard.edu> 9/19/2005 10:41:09 AM >>> Hi Flowers, How are people approaching the sorting of lentivirus transduced cells? Thank you for any advice, Maris Maris A. Handley Dana-Farber Cancer Institute HIM 432, 77 Avenue Louis Pasteur Boston, MA 02115 617-632-4490Received on Tue Sep 20 12:58:00 2005
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