A collaborator in my lab is wondering how to detect cell death other than by using dyes. In his experiments, he can detect dead cells using staining methods with annexin V/PI, eosin Y and other exclusion dyes. However, cells are found dead only 30 min after the beginning of the treatment, a time-point that appears to early for any standard apoptotic pathways. Mitochondria membrane potential change is also detected. Cells are only treated with a lectin, so mechanical action on cell membrane is excluded. Any suggestion to confirm/infirm the death of the cells? Thanks, ----------------------------------------------------------------------- Michel Theron, BSc MSc MSc Research Assistant Lei's Lab Department of Microbiology and Immunology National Cheng Kung University Medical College 1 Ta-Hsueh Road 701 Tainan Taiwan, R.O.C. Tel: +886 (0)6 235 3535 ext. 5644 ___________________________________________________________ Yahoo! Messenger - NEW crystal clear PC to PC calling worldwide with voicemail http://uk.messenger.yahoo.comReceived on Mon Sep 12 13:38:00 2005
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