in vivo injection of Annexin-V

Dear list and ISAC members:
	An investigator at our lab is interested in the following procedure to 
study muscular dystrophy in mouse models. He has a good idea but the 
reagents are a problem, mainly that there is no way to quantify the 
amount of annexin-V. I suggested we put this out to the experts and see 
if any of you have any leads that may help us. Thanks in advance for 
your help.
	Here is his message:



I am interested in using an Annexin-V biotin kit for in vivo injection 
into mouse, followed by harvesting of skeletal muscle and embedding in 
parafin to detect apoptosis.  I am planning to follow published 
techniques  which use 25mg/kg body weight.  I contacted Molecular 
Probes in the United States to find out what the concentration of the 
Annexin-V is in their kit (A13204) to determine whether there is enough 
Annexin-V in one kit to do the number of injections I need for my 
experiments.  Unfortunately, they told me that the information on the 
concentration of Annexin-V in their product is proprietary information 
that they cannot reveal, so their product is useless to  me.

Does anyone have any information on concentrations of Annexin-V biotin 
labeling kits that I could use at the required concentrations for this 
in vivo injection study?


Thanks,

Roger B. Sher, PhD
The Jackson Laboratory
600 Main Street
Bar Harbor, ME 04609


Ted Duffy
Flow Cytometry
The Jackson Laboratory
600 Main Street
Bar Harbor, Maine 04609-1500
(207) 288-6192