Re: FACSAria

We have used a FACSAria in the Vanderbilt core to perform difficult
antigen-specific human B cell sorting experiments, using single-cell, bulk,
and four way formats. It took us several months to works through proper
pressures and other parameters, but we are routinely obtaining very high
purity sorts with high viability, on a repetitive basis. Initially we had
some instrument difficulties with poor QC on the cuvettes, and bad nozzles,
but once we got a decent cuvette in (might have been our 4th or 5th) the
machine settled down and started yielding excellent sorts. Our sorting
success has been heavily dependent on the technical expertise of our core
manager.

Jim Crowe



On 8/23/05 8:47 AM, "John Kearney" <jfk@uab.edu> wrote:

> We have had a FACSAria in the lab for > 2yrs. Well actually two since the
> first one was replaced. We continue to be plagued by the inability to sort
> accurately and consistently  acceptably clean populations of mouse T and B
> cells on this machine, particularly if they are small subsets. Despite
> various fixes and technical advice this machine has been a big
> disappointment given the pre-release claims for this BD machine. I was
> wondering if other FACSAria users have had similar problems and if so were
> they surmountable? Thanks John
> John F Kearney
> Professor of Microbiology
> 378 Tumor Institute
> University of Alabama at Birmingham
> Birmingham Al 35294
> jfk@uab.edu 
> http://www.uab.edu/luckielab/
> Ph 205 934 6557 
> FAX 205 934 1875 
> Courier 
> 378 Tumor Institute
> 1824 6th Ave South
> Birmingham Al 35294
> 
>