Overall, I've found my Aria to be the best sorter in my lab (1 Aria, 2 DiVas, 1 SE). However, I am very particular when choosing my nozzles. In the old days, they used to send them 2-5 at a time, and I could count on getting one to two tips that sorted well and the rest that were basically useless. The machine would eat the tips over the course of a few months, of course until I performed the Parks mod and then the BD upgrade. I've been sent several ceramic tips, but none of them seem to perform well. I am currently using three covetously horded 100um gold tips and getting excellent sort performance even at the 20 psi where I do most of my sorting. David Adams University of Michigan Flow Cytometry Core >>> John Kearney <jfk@uab.edu> 08/23/05 9:47 AM >>> We have had a FACSAria in the lab for > 2yrs. Well actually two since the first one was replaced. We continue to be plagued by the inability to sort accurately and consistently acceptably clean populations of mouse T and B cells on this machine, particularly if they are small subsets. Despite various fixes and technical advice this machine has been a big disappointment given the pre-release claims for this BD machine. I was wondering if other FACSAria users have had similar problems and if so were they surmountable? Thanks John John F Kearney Professor of Microbiology 378 Tumor Institute University of Alabama at Birmingham Birmingham Al 35294 jfk@uab.edu http://www.uab.edu/luckielab/ Ph 205 934 6557 FAX 205 934 1875 Courier 378 Tumor Institute 1824 6th Ave South Birmingham Al 35294 ********************************************************** Electronic Mail is not secure, may not be read every day, and should not be used for urgent or sensitive issues.Received on Wed Aug 24 15:38:00 2005
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