Once again thank you very much for all your input. This is a great mail list with lots of interesting opinions. I include a word file with a summary of the answers. I finally chose the concentration of directly conjugated mouse antibody with the highest signal/noise ratio (while blocking with mouse serum), and used as a comparison IC at the same concentration. --------------------------------- Correo Yahoo! Comprueba qué es nuevo, aquí http://correo.yahoo.es This attachment - 'Titering Antibodies and isotype controls.doc' - 66.05 KBytes - can be viewed at http://www.cyto.purdue.edu/MD-parts/087daac8e02facf4b7dfa87079baf77b1cd729ce.docReceived on Fri Aug 19 13:18:00 2005
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