Like most Abs, tetramers have to be titrated when staining PBMC. Recommendations from commercial vendors indicate that one should use "X" dilution per 10^6 cells. Does that mean 10^6 positive cells (in the unlikely event that all cells would be tetramer positive), or does it mean 10^6 total PBMC with a small percentage of PBMC likely to be tetramer-positive? Tetramers are so expensive that I don't want to waste the reagent if I'm going to stain 40 x 10^6 PBMC when only 1.5% of them will be positive. Any thoughts? -Doug -- =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=- Douglas F. Lake, Ph.D. University of Arizona Cancer Center, Room 4919 1515 N. Campbell Ave P.O. Box 245024 Tucson, AZ 85724 Office phone: 520/626-4101 Lab phone: 520/626-4102 FAX: 520/626-3754 =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-Received on Tue Aug 16 13:38:00 2005
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