Fluorescence changes in cells grown with serum in cell cycle analysis

From: Guillermo Palao <gpbnas@yahoo.es>
Date: Wed Jul 27 2005 - 05:59:47 EST
Hello everybody,
I have been analysing cell cycle with FACSCAN by staining methanol-permeabilized
fibroblasts with propidium iodide. When comparing cells grown in the presence of serum
with those without serum, besides expected changes in % cells in Go-1 vs S-G2-M I found a
global decrease in FL2 fluorescence in samples of cells grown with serum. Does anybody
know why this may be happening?
Thanks in advance,
Guillermo Palao
Laboratorio de Reumatología
Centro de Investigación
Hospital 12 de Octubre
Madrid 




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Received on Wed Jul 27 11:38:00 2005

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